Protease susceptibility of human A influenza virus polypeptides.
Identifieur interne : 002703 ( Main/Exploration ); précédent : 002702; suivant : 002704Protease susceptibility of human A influenza virus polypeptides.
Auteurs : L R Sabina ; M E Ewasyshyn ; R. Hills ; D A McleodSource :
- Acta virologica [ 0001-723X ] ; 1981.
Descripteurs français
- KwdFr :
- Animaux, Dénaturation des protéines, Embryon de poulet, Peptide hydrolases (métabolisme), Peptides (métabolisme), Phospholipase D (métabolisme), Protéines virales (métabolisme), Trypsine (métabolisme), Virus de la grippe A (immunologie), Virus de la grippe A (métabolisme), Électrophorèse sur gel de polyacrylamide.
- MESH :
- immunologie : Virus de la grippe A.
- métabolisme : Peptide hydrolases, Peptides, Phospholipase D, Protéines virales, Trypsine, Virus de la grippe A.
- Animaux, Dénaturation des protéines, Embryon de poulet, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Peptide Hydrolases, Peptides, Phospholipase D, Trypsin, Viral Proteins.
- immunology : Influenza A virus.
- metabolism : Influenza A virus.
- Animals, Chick Embryo, Electrophoresis, Polyacrylamide Gel, Protein Denaturation.
Abstract
The proteolytic susceptibility of polypeptides of four antigenically distinct subtypes of influenza a virus strains of human origin was studied. The extent of degradation of polypeptide molecules of strains A/PR/8/34 (H0N1) (PR), A/FM/1/47 (H1N1), A/Singapore/1/57 (H2N2) and A/Hong Kong/8/68 (H3N2), assessed by densitometry of gels after sodium dodecylsulfate polyacrylamide gel electrophoresis was variable by treatment with trypsin. Also, sequential treatment of PR strain initially with phospholipase D followed by proteases of different specificities suggested differences in susceptibility of surface and internal polypeptide molecules. The significance of these results is discussed.
PubMed: 6115561
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000576
- to stream PubMed, to step Curation: 000576
- to stream PubMed, to step Checkpoint: 000496
- to stream Ncbi, to step Merge: 001160
- to stream Ncbi, to step Curation: 001160
- to stream Ncbi, to step Checkpoint: 001160
- to stream Main, to step Merge: 002844
- to stream Main, to step Curation: 002703
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Protease susceptibility of human A influenza virus polypeptides.</title><author><name sortKey="Sabina, L R" sort="Sabina, L R" uniqKey="Sabina L" first="L R" last="Sabina">L R Sabina</name></author><author><name sortKey="Ewasyshyn, M E" sort="Ewasyshyn, M E" uniqKey="Ewasyshyn M" first="M E" last="Ewasyshyn">M E Ewasyshyn</name></author><author><name sortKey="Hills, R" sort="Hills, R" uniqKey="Hills R" first="R" last="Hills">R. Hills</name></author><author><name sortKey="Mcleod, D A" sort="Mcleod, D A" uniqKey="Mcleod D" first="D A" last="Mcleod">D A Mcleod</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1981">1981</date><idno type="RBID">pubmed:6115561</idno><idno type="pmid">6115561</idno><idno type="wicri:Area/PubMed/Corpus">000576</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000576</idno><idno type="wicri:Area/PubMed/Curation">000576</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000576</idno><idno type="wicri:Area/PubMed/Checkpoint">000496</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000496</idno><idno type="wicri:Area/Ncbi/Merge">001160</idno><idno type="wicri:Area/Ncbi/Curation">001160</idno><idno type="wicri:Area/Ncbi/Checkpoint">001160</idno><idno type="wicri:doubleKey">0001-723X:1981:Sabina L:protease:susceptibility:of</idno><idno type="wicri:Area/Main/Merge">002844</idno><idno type="wicri:Area/Main/Curation">002703</idno><idno type="wicri:Area/Main/Exploration">002703</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Protease susceptibility of human A influenza virus polypeptides.</title><author><name sortKey="Sabina, L R" sort="Sabina, L R" uniqKey="Sabina L" first="L R" last="Sabina">L R Sabina</name></author><author><name sortKey="Ewasyshyn, M E" sort="Ewasyshyn, M E" uniqKey="Ewasyshyn M" first="M E" last="Ewasyshyn">M E Ewasyshyn</name></author><author><name sortKey="Hills, R" sort="Hills, R" uniqKey="Hills R" first="R" last="Hills">R. Hills</name></author><author><name sortKey="Mcleod, D A" sort="Mcleod, D A" uniqKey="Mcleod D" first="D A" last="Mcleod">D A Mcleod</name></author></analytic><series><title level="j">Acta virologica</title><idno type="ISSN">0001-723X</idno><imprint><date when="1981" type="published">1981</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chick Embryo</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Influenza A virus (immunology)</term><term>Influenza A virus (metabolism)</term><term>Peptide Hydrolases (metabolism)</term><term>Peptides (metabolism)</term><term>Phospholipase D (metabolism)</term><term>Protein Denaturation</term><term>Trypsin (metabolism)</term><term>Viral Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Dénaturation des protéines</term><term>Embryon de poulet</term><term>Peptide hydrolases (métabolisme)</term><term>Peptides (métabolisme)</term><term>Phospholipase D (métabolisme)</term><term>Protéines virales (métabolisme)</term><term>Trypsine (métabolisme)</term><term>Virus de la grippe A (immunologie)</term><term>Virus de la grippe A (métabolisme)</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Peptide Hydrolases</term><term>Peptides</term><term>Phospholipase D</term><term>Trypsin</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Peptide hydrolases</term><term>Peptides</term><term>Phospholipase D</term><term>Protéines virales</term><term>Trypsine</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chick Embryo</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Protein Denaturation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Dénaturation des protéines</term><term>Embryon de poulet</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The proteolytic susceptibility of polypeptides of four antigenically distinct subtypes of influenza a virus strains of human origin was studied. The extent of degradation of polypeptide molecules of strains A/PR/8/34 (H0N1) (PR), A/FM/1/47 (H1N1), A/Singapore/1/57 (H2N2) and A/Hong Kong/8/68 (H3N2), assessed by densitometry of gels after sodium dodecylsulfate polyacrylamide gel electrophoresis was variable by treatment with trypsin. Also, sequential treatment of PR strain initially with phospholipase D followed by proteases of different specificities suggested differences in susceptibility of surface and internal polypeptide molecules. The significance of these results is discussed.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Ewasyshyn, M E" sort="Ewasyshyn, M E" uniqKey="Ewasyshyn M" first="M E" last="Ewasyshyn">M E Ewasyshyn</name><name sortKey="Hills, R" sort="Hills, R" uniqKey="Hills R" first="R" last="Hills">R. Hills</name><name sortKey="Mcleod, D A" sort="Mcleod, D A" uniqKey="Mcleod D" first="D A" last="Mcleod">D A Mcleod</name><name sortKey="Sabina, L R" sort="Sabina, L R" uniqKey="Sabina L" first="L R" last="Sabina">L R Sabina</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002703 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002703 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:6115561
|texte= Protease susceptibility of human A influenza virus polypeptides.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:6115561" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |